Journal: Journal of visualized experiments : JoVE

Article Title: Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models

doi: 10.3791/65561

Figure Lengend Snippet: Materials and reagents. A list of all reagents and equipment with any available catalog numbers. Note that there are some materials that have related items that can act as substitutes.

Article Snippet: Amyloid-β (1-42, HiLyte Fluor647, 0.1 mg) , AnaSpec , AS-64161 , Other fluorescent conjugates available.

Techniques: Microscopy, Imaging, Cell Culture, Software, Inverted Microscopy, Live Cell Imaging

Journal: Molecules and Cells

Article Title: ER stress and unfolded protein response (UPR) signaling modulate GLP-1 receptor signaling in the pancreatic islets

doi: 10.1016/j.mocell.2023.12.002

Figure Lengend Snippet: Acute ER stress triggers GLP-1R signaling use of Gq. (A-D) Isolated mouse islets were pretreated with tunicamycin (Tm, 1 μg/ml, 8 hours) and further administered with MDL-12330A (MDL, adenylyl cyclase inhibitor, 10 μM) (A, B) or YM-254890 (YM, Gq inhibitor, 200 nM) (C, D) along with exendin-4 (Ex4, 50 nM) and high glucose (Glc, 17 mM) for an additional 1 hour. (A, C) Insulin secretion and (B, D) its fold change. Data are presented as means ± SEM (n = 4). Statistical analyses were performed using either 1-way ANOVA followed by Holm-Sidak’s test (A, C) or unpaired t-tests with Welch’s correction (B, D). ns, non-significance. * P < .05, ** P < .01, *** P < .001.

Article Snippet: Exendin-4 (Ex4) was obtained from Merck (E7144), whereas IXA4 was purchased from DC Chemicals (Cat no. DC51012).

Techniques: Isolation

Journal: Molecules and Cells

Article Title: ER stress and unfolded protein response (UPR) signaling modulate GLP-1 receptor signaling in the pancreatic islets

doi: 10.1016/j.mocell.2023.12.002

Figure Lengend Snippet: 4-PBA, a chemical chaperone, promotes GLP-1R agonist-induced insulin secretion and GLP-1R’s Gq utilization in ER stress-experiencing and db/db islets. (A, B) Islet insulin secretion. Isolated lean mouse islets were sequentially treated with 4-PBA (2.5 mM, 24 hours), tunicamycin (1 μg/ml, 8 hours), and high glucose (Glc, 17 mM, 1 hour) with or without Ex4 (50 nM), YM (200 nM), and MDL (10 μM). (C-E) Islets were isolated from 12-week-old db/db mice and then pretreated with 4-PBA (2.5 mM, 24 hours) before high glucose with indicated reagents. (C) Insulin secretion and (D, E) its fold change. Data are presented as means ± SEM (n = 4). Statistical analyses were performed using either unpaired t-tests (A, D, E) or 1-way ANOVA (B, C). ns, non-significance. * P < .05, ** P < .01, *** P < .001.

Article Snippet: Exendin-4 (Ex4) was obtained from Merck (E7144), whereas IXA4 was purchased from DC Chemicals (Cat no. DC51012).

Techniques: Isolation

Journal: Journal of the Endocrine Society

Article Title: Glucagon-Like Peptide 1 and Atrial Natriuretic Peptide in a Female Mouse Model of Obstructive Pulmonary Disease

doi: 10.1210/jendso/bvz034

Figure Lengend Snippet: ANP and EX-4 both exerted bronchodilating effects on isolated bronchi of mice. Concentration-relaxation curves induced by ANP and EX-4 on bronchial smooth muscle tissue. Values are expressed relative to the maximal effect of SNP (0.1 mM), which was added at the end of the experiment. Data are shown as means ± SEM. A, Relaxation induced by ANP (0.001-1 mM) in healthy mice. B, Relaxation induced by EX-4 (0.001-1 mM) in healthy mice. C, The effect of ANP on tissue from COPD mice compared to healthy mice. D, The effect of EX-4 on COPD compared to healthy mice. Data from A and B were analyzed by 1-way ANOVA followed by Bonferroni post hoc test comparing each concentration to 0.001 mM, which was considered as zero effect. Data from C and D were analyzed by 2-way ANOVA followed by Sidak post hoc test comparing the responses of COPD and healthy mice at each concentration. * P < .05, ** P < .01.

Article Snippet: ANP (A8208, Sigma-Aldrich) and exendin-4 (EX-4) (Caslo) for wire myography was dissolved in double-distilled H 2 O. EX-4 is a 39 AA peptide with 50% homology to GLP-1 ( ) that is not influenced by variable protein binding.

Techniques: Isolation, Concentration Assay

Journal: Journal of the Endocrine Society

Article Title: Glucagon-Like Peptide 1 and Atrial Natriuretic Peptide in a Female Mouse Model of Obstructive Pulmonary Disease

doi: 10.1210/jendso/bvz034

Figure Lengend Snippet: Direct effects of ANP and Ex-4 on smooth muscles of isolated mouse bronchi, including nonresponders. Concentration-relaxation curves induced by ANP and EX-4 on bronchial smooth muscle tissue. Relaxation values are expressed as a percentage of the relaxation seen with a maximally effective concentration of SNP (0.1 mM) added at the end of the experiment. Data are shown as means ± SEM. A, The effect of ANP on tissue from COPD compared to healthy mice. B, The effect of EX-4 on COPD mice compared to healthy mice. C, Example of myograph signals from tissue sections responding to ANP and D, not responding to ANP, but to SNP.

Article Snippet: ANP (A8208, Sigma-Aldrich) and exendin-4 (EX-4) (Caslo) for wire myography was dissolved in double-distilled H 2 O. EX-4 is a 39 AA peptide with 50% homology to GLP-1 ( ) that is not influenced by variable protein binding.

Techniques: Isolation, Concentration Assay